Estrogen receptor gene 1 expression in caprine and its effect on fertility

Authors

  • A. K. Goel Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
  • P. K. Rout GGB Division, Central Institute for Research on goats (CIRG), Makhdoom, Farah- 281122, Mathura, Uttar Pradesh, India
  • S. D. Kharche Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
  • S. K. Jindal Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
  • S. Kumar Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
  • S. Saraswat Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
Abstract:

The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene (ESR1) in Barbari bucks (fertile and non-fertile) identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction (RT-PCR). The expression pattern of ESR1 gene was analyzed by RT-PCR (Roche LC-480). Relative quantification by RT-PCR indicated that the ESR1 gene expression showed more fold in fertile bucks as compared to non-fertile.

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Journal title

volume 17  issue 3

pages  207- 209

publication date 2016-09-19

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